Avidin-Biotin Interactions
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For researchers today, the avidin-biotin interaction is among the most widely exploited among biomedical scientists. Despite its popularity, avidin-biotin methodology suffers from a lack of clarity associated with popular, low cost, prefabricated kits used by many in the field. In Avidin-Biotin Interactions: Methods and Applications, leaders in avidin-biotin methodology share their knowledge relating to the application of the extremely powerful interaction between the protein avidin or its homologues and the vitamin biotin and some of its homologues. Although avidin-biotin based kits based have been produced for quite some time and remain a widely used methodology, many of the chapters in this text describe situations in which the previous methodology failed due to some factor, and the authors developed new and better ways for the avidin-biotin system to be utilized. With excellent descriptions of laboratory protocols written by expert researchers, this volume is equally perfect for the student or the professional laboratory scientist. Each chapter in Avidin-Biotin Interactions: Methods and Applications contains not only excellent descriptions of protocols for the lab but also the experience of researchers who used specific methodology, found a problem with that methodology in a new setting, and ultimately devised ways to minimize or obviate the limitations of the technology.
Preface vContributors xiPreparation of Avidin Conjugates Rosaria P. Haugland Mahesh K. Bhalgat 1Coupling of Antibodies With Biotin Rosaria P. Haugland Wendy W. You 13Optimization of Detection and Quantification of Proteins on Membranes in Very High and Very Low Abundance Using Avidin and Streptavidin Sara R. Zwart Brandon J. Lewis 25Use of the Avidin (Imino) Biotin System as a General Approach to Affinity Precipitation Ruth Freitag Frank Hilbrig 35Capturing Biotinylated Proteins and Peptides by Avidin Functional Affinity Electrophoresis Bao-Shiang Lee Sangeeth Krishnanchettiar Syed Salman Lateef Shalini Gupta 51Functionality Screen of Streptavidin Mutants by Non-Denaturing SDS-PAGE Using Biotin-4-Fluorescein Nicolas Humbert Thomas R. Ward 63Application of Biotin-4-Fluorescein in Homogeneous Fluorescence Assays for Avidin, Streptavidin, and Biotin or Biotin Derivatives Andreas Ebner Markus Marek Karl Kaiser Gerald Kada Christoph D. Hahn Bernd Lackner Hermann J. Gruber 73Quantitative Recovery of Biotinylated Proteins from Streptavidin-Based AffinityChromatography Resins Christoph Rosli Jascha-N. Rybak Dario Neri Giuliano Elia 89High-Yield Production and Purification of Recombinant T7-Tag Mature Streptavidin in Glucose-Stressed E. coli Nicolas Humbert Peter Schurmann Andrea Zocchi Jean-Marc Neuhaus Thomas R. Ward 101Endogenous Biotin in Rat Brain: Implications for False-Positive Results With Avidin-Biotin and Streptavidin-Biotin Techniques Bruce E. McKay Michael L. Molineux Ray W. Turner 111Pseudo-Immunolabelling With the Avidin-Biotin-Peroxidase Complex due to the Presence of Endogenous Biotin in the Retina Willem Kamphuis Jan Klooster 129Use of Synthetic Peptides for Identifying Biotinylation Sites in Human Histones Gabriela Camporeale Yap Ching Chew Alice Kueh Gautam Sarath Janos Zempleni 139Detection and Quantitation of the Activity of DNA Methyltransferases Using a Biotin/Avidin Microplate Assay Kirsten Liebert Albert Jeltsch 149Anti-Biotin Antibodies Offer Superior Organelle-Specific Labelling of Mitochondria Over Avidin or Streptavidin Elisabeth D. Coene Michael K. Shaw David J. Vaux 157Mammalian Lectin as Tool in Glycochemistry and Histochemistry With Relevance for Diagnostic Procedure Karel Smetana, Jr. Sabine Andre 171A Multiplex Approach to Isotyping Antigen-Specific Antibodies Using Biotinylated Antigen/Streptavidin-Phycoerythrin Krista McCutcheon 187Biotin-Protein Bond: Instability and Structural Modification to Provide Stability for In Vivo Applications Donald M. Mock Anna Bogusiewicz 209Index 221
