Forensic Applications of High Performance Liquid Chromatography
Chromatography has many roles in forensic science, ranging from toxicology to environmental analysis. In particular, high-performance liquid chromatography (HPLC) is a primary method of analysis in many types of laboratories. Maintaining a balance between practical solutions and the theoretical considerations involved in HPLC analysis, Forensic Applications of High Performance Liquid Chromatography uses real-life examples likely to be found within a forensic science laboratory to explain HPLC...
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Chromatography has many roles in forensic science, ranging from toxicology to environmental analysis. In particular, high-performance liquid chromatography (HPLC) is a primary method of analysis in many types of laboratories. Maintaining a balance between practical solutions and the theoretical considerations involved in HPLC analysis, Forensic Applications of High Performance Liquid Chromatography uses real-life examples likely to be found within a forensic science laboratory to explain HPLC from a forensic perspective.Focusing chiefly on the reverse phase HPLC mode of separation, this volume examines:The history of HPLC and the theory behind the separation processThe requirements for successful analysis and best practice tipsThe modes of separation and detection most appropriate for forensic science applicationsHPLC method development and evaluationThe quality aspects of laboratory operationTroubleshooting HPLC systems and analysesApplications of HPLC within the field of forensic scienceDesigned as a textbook for university students studying analytical chemistry, applied chemistry, forensic chemistry, or other courses with an element of HPLC within the course curriculum, this volume is also an invaluable guide for those in the early stages of their forensic analysis careers.An instructor’s manual with lecture slides, test bank, objectives, and exercises is available with qualifying course adoption
1 Introduction to HPLC 12 Basic Principles of HPLC 53 Mobile Phase Preparation, the Use of Buffers, and Sample Preparation 454 Modes of Separation 775 Detection Systems 876 Method Development in Reversed Phase HPLC 1157 System Suitability 1458 Qualification, Validation, and Verification 1499 Quality Systems 17710 Troubleshooting HPLC Systems 18711 Forensic Applications of HPLC 213Glossary 235Index 241
