Gene Cloning and DNA Analysis: An Introduction
Known world-wide as the standard introductory text to this important and exciting area, the sixth edition of Gene Cloning and DNA Analysis addresses new and growing areas of research whilst retaining the philosophy of the previous editions. Assuming the reader has little prior knowledge of the subject, its importance, the principles of the techniques used and their applications are all carefully laid out, with over 250 clearly presented four-colour illustrations.\ In addition to a number of...
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Known world-wide as the standard introductory text to this important and exciting area, the sixth edition of Gene Cloning and DNA Analysis addresses new and growing areas of research whilst retaining the philosophy of the previous editions. Assuming the reader has little prior knowledge of the subject, its importance, the principles of the techniques used and their applications are all carefully laid out, with over 250 clearly presented four-colour illustrations.In addition to a number of informative changes to the text throughout the book, the final four chapters have been significantly updated and extended to reflect the striking advances made in recent years in the applications of gene cloning and DNA analysis in biotechnology.
1Why gene cloning and DNA analysis are important32Vectors for gene cloning : plasmids and bacteriophages143Purification of DNA from living cells284Manipulation of purified DNA545Introduction of DNA into living cells876Cloning vectors for E. coli1077Cloning vectors for eukaryotes1328How to obtain a clone of a specific gene1589The polymerase chain reaction18110Studying gene location and structure19911Studying gene expression and function22012Studying genomes25213Production of protein from cloned genes27714Gene cloning and DNA analysis in medicine30215Gene cloning and DNA analysis in agriculture32316Gene cloning and DNA analysis in forensic science and archaeology346
