Phage Display in Biotechnology and Drug Discovery
The first and only guide to showcase the impact of phage display technology on drug discovery, this reference details the theories, principles, and methods impacting the field and demonstrates applications for peptide phage display, protein phage display, and the development of novel antibodies. Highlighting the current and future role of phage display in the development of protein therapeutics, this book provides a comprehensive overview that will prove invaluable to anyone researching...
Search in google:
The first and only guide to showcase the impact of phage display technology on drug discovery, this reference details the theories, principles, and methods impacting the field and demonstrates applications for peptide phage display, protein phage display, and the development of novel antibodies. Highlighting the current and future role of phage display in the development of protein therapeutics, this book provides a comprehensive overview that will prove invaluable to anyone researching recombinant antibodies. Doody Review Services Reviewer:Eugene A Davidson, PhD(Georgetown University School of Medicine)Description:This book provides a set of reviews on various aspects of phage display technology. Coverage progresses from general introductory and background material to current applications.Purpose:The goal is to provide an overview of the present status of phage display technology (principles as well as applications). Given the high interest in combinatorial methods, this is a worthy goal that, in general, is achieved.Audience:Phage display is one of several combinatorial approaches presently used. The target audience includes scientists working in the area and those wishing to familiarize themselves with it. The editor has assembled a diverse and competent set of contributors. Features:Phage display, first described about 20 years ago, was the first of the biotechnology-based combinatorial techniques available (a problem addressed eons earlier in the development of antibody responses). The diversity of expressed products derived from random DNA sequences is obviously huge and the corresponding implementation of high throughput screening methods allows for their exploitation for pharmaceutical development. This book provides a series of reviews on phage display techniques with an emphasis on their utility in drug development. The 17 chapters begin with a general discussion of the filamentous bacteriophage and useful vectors, library construction, and screening methods. Several chapters are devoted to specific applications such as antibody engineering, protein-protein interactions, and enzymatic optimization. Each section is accompanied by a comprehensive bibliography allowing easy access to primary sources. Although several similar treatises have appeared recently, the focus here is on the applications of phage display to therapeutic issues. This book will be of value to those working in this area as well as to investigators wishing an introduction to a powerful and increasingly used technology.Assessment:This is a generally well-written set of articles on a well-known technique. Those not directly in the field can broaden their understanding of the breadth of applicability of this method. Although several similar books have recently appeared (O'Brien and Aitken, Antibody Phage Display: Methods and Protocols (Humana Press, 2002); Clackson and Lowman, Phage Display: A Practical Approach (Oxford University Press, 2004)), the organized coverage of this book is useful for almost any reader.
Contents Filamentous Bacteriophage Structure and Biology, D.J. Rodi, S. Mandava, and L. Makowski Introduction Taxonomy and Genetics Viral Gene Products Structure of the Virion Filamentous Bacteriophage Life Cycle Phage Library Diversity Biological Bottlenecks: Sources of Library Censorship Quantitative Diversity Estimation Improved Library Construction Vectors and Modes of Display, V.A. Petrenko and G.P. Smith Introduction Most Display Vectors are Based on Filamentous Phage General Cloning Vectors Based on Filamentous Phage Classification of Filamentous Phage Display Systems Phage f1—The First Phage-Display Vector Low DNA Copy Number Display Vectors Based on Fd-Tet Diversity of Type 3 Vectors Type 8 Vectors: First Lessons Mosaic Display in Type nn Systems Mosaic Display in Phagemid Systems Vectors for C-Terminal Display Phage Proteins as Constraining Scaffolds Conclusion Methods for the Construction of Phage-Displayed Libraries, F.A. Fellouse and G. Pal Introduction Oligonucleotide-Directed Mutagenesis Random Mutagenesis Combinatorial Infection and Recombination DNA Shuffling Selection and Screening Strategies, M.S. Dennis Introduction General Considerations The Selection Process Selections Methods Phage Library for Developing Antibody-Targeted Diagnostics and Vaccines, N.E. van Houten and J.K. Scott Introduction Phage-Display Libraries as Tools for Epitope Discovery Diagnostics Phage Libraries for Epitope Mapping Phage Display Libraries for Vaccine Development Developing Immunogens From Peptide Leads Summary Conclusion Abbreviations Exploring Protein—Protein Interactions Using Peptide Libraries Displayed on Phage, K. Deshayes Introduction Extracellular Protein—Protein Interactions Intracellular Protein—Protein Interactions Conclusions Substrate Phage Display, S. Ohkubo Overview Introduction The Concept of Substrate Phage Display Application of Substrate Phage Display to Cancer Research Conclusions Mapping Intracellular Protein Networks, Z. Han, E. Karatan, and B.K. Kay Introduction Domain-Mediated Interactions Nondomain Mediated Protein—Protein Interactions Software for Identifying Candidate Interacting Partners Analyzing Predicted Interactions Relevance to Biotechnology and Drug Discovery High Throughput and High Content Screening Using Peptides, R.O. Carlson, R. Hyde-DeRuyscher, and P.T. Hamilton Introduction Peptides as Enzyme Inhibitors Peptides as Conformational Probes Summary Engineering Protein Folding and Stability, M. Tuna and D.N. Woolfson Protein Redesign and Design Early Combinatorial Studies Aimed at Repacking the Cores of Proteins Phage Display in Engineering Protein Stability A Worked Example: Repacking the Hydrophobic Core of Ubiquitin Studies that Build on the Original Methods Summary Identification of Natural Protein—Protein Interactions with cDNA Libraries, R. Crameri, C. Rhyner, M. Weichel, S. Flückiger, and Z. Konthur Overview Introduction Cloning Vectors Display of cDNA Libraries on Phage Surface Problems Associated with the Display of cDNA Libraries on Phage Surface Adaptability of Phage Display to High-Throughput Screening Technology Conclusions Mapping Protein Functional Epitopes, S.K. Avrantinis and G.A. Weiss Introduction Single Point Alanine Mutagenesis Combinatorial Site-Specific Mutagenesis Other Approaches to Phage-Displayed Functional Epitope Mapping Conclusion Selections for Enzymatic Catalysts, J. Bertschinger, C. Heinis, and D. Neri Introduction Selection Methods Discussion Antibody Humanization and Affinity Maturation Using Phage Display, J.S. Marvin and H.B. Lowman Introduction Humanization Using Phage Display In Vitro Affinity Maturation of Antibodies Emerging Approaches Conclusions Antibody Libraries from Immunized Repertoires, J.D. Berry and M. Popkov Introduction Immune Antibody Library Construction Immune Antibody Library Selection The Future Acknowledgements Naïve Antibody Libraries from Natural Repertoires, C.L. Dobson, R.R. Minter, and C.P. Hart-Shorrock Introduction Construction of Naïve Libraries Applications of Naïve Libraries Synthetic Antibody Libraries, F.A. Fellouse and S.S. Sidhu Introduction The Scripps Research Institute The Medical Research Council Morphosys Genentech Conclusions Index
\ From The CriticsReviewer: Eugene A Davidson, PhD(Georgetown University School of Medicine)\ Description: This book provides a set of reviews on various aspects of phage display technology. Coverage progresses from general introductory and background material to current applications.\ Purpose: The goal is to provide an overview of the present status of phage display technology (principles as well as applications). Given the high interest in combinatorial methods, this is a worthy goal that, in general, is achieved.\ Audience: "Phage display is one of several combinatorial approaches presently used. The target audience includes scientists working in the area and those wishing to familiarize themselves with it. The editor has assembled a diverse and competent set of contributors. "\ Features: Phage display, first described about 20 years ago, was the first of the biotechnology-based combinatorial techniques available (a problem addressed eons earlier in the development of antibody responses). The diversity of expressed products derived from random DNA sequences is obviously huge and the corresponding implementation of high throughput screening methods allows for their exploitation for pharmaceutical development. This book provides a series of reviews on phage display techniques with an emphasis on their utility in drug development. The 17 chapters begin with a general discussion of the filamentous bacteriophage and useful vectors, library construction, and screening methods. Several chapters are devoted to specific applications such as antibody engineering, protein-protein interactions, and enzymatic optimization. Each section is accompanied by a comprehensive bibliography allowing easy access to primary sources. Although several similar treatises have appeared recently, the focus here is on the applications of phage display to therapeutic issues. This book will be of value to those working in this area as well as to investigators wishing an introduction to a powerful and increasingly used technology.\ Assessment: This is a generally well-written set of articles on a well-known technique. Those not directly in the field can broaden their understanding of the breadth of applicability of this method. Although several similar books have recently appeared (O'Brien and Aitken, Antibody Phage Display: Methods and Protocols (Humana Press, 2002); Clackson and Lowman, Phage Display: A Practical Approach (Oxford University Press, 2004)), the organized coverage of this book is useful for almost any reader.\ \
