Practical HPLC Method Development
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This revision brings the reader completely up to date on the evolving methods associated with increasingly more complex sample types analyzed using high-performance liquid chromatography, or HPLC. The book also incorporates updated discussions of many of the fundamental components of HPLC systems and practical issues associated with the use of this analytical method. This edition includes new or expanded treatments of sample preparation, computer assisted method development, as well as biochemical samples, and chiral separations. Booknews Details current knowledge on method development for high-performance liquid chromatography (HPLC). This second edition offers increased focus on specialized applications for separating analytes such as biological samples and mixtures of enantiomers, and expanded material on computer-assisted method development, HPLC column technology, and biochemical and chiral applications, integrating background and practical material from Snyder and Kirkland's "Introduction to Modern Liquid Chromatography". For scientists and students beginning work with HPLC as well as experienced analysts. Annotation c. by Book News, Inc., Portland, Or.
PrefaceGlossary of Symbols and Terms1Getting Started12Basics of Separation213Detection Sensitivity and Selectivity594Sample Preparation1005The Column1746Non-ionic Samples: Reversed- and Normal-Phase HPLC2337Ionic Samples: Reversed-Phase, Ion-Pair, and Ion-Exchange HPLC2928Gradient Elution3509Systematic Approach to the Reversed-Phase Separation of Regular Samples40210Computer-Assisted Method Development43911Biochemical Samples: Proteins, Nucleic Acids, Carbohydrates, and Related Compounds47912Chiral Separations53713Preparative HPLC Separation61614Quantitation (Including Trace Analysis)64315Completing the Method: Validation and Transfer685App. IPlate Number and Resolution714App. IIProperties of Solvents Used in HPLC721App. IIIRetention in Reversed-Phase and Normal-Phase HPLC as a Function of Sample Molecular Structure729App. IVPreparing Buffered Mobile Phases735App. VCharacterizing the Differences Among C[subscript 8] or C[subscript 18] Reversed-Phase Columns from Different Suppliers740App. VIAdjusting Mobile-Phase Water Content for Normal-Phase HPLC744Index747
