Protein Purification 2e
Praise for the First Edition of Protein Purification:\ "Contains much useful information for both the novice in protein purification and the experienced practitioner . . . [an] excellent practical manual." -Trends in Analytical Chemistry.\ "[This book is] easy to read and gives a comprehensive description of all major methods of protein purification . . . a much welcomed source of information for protein biochemists." -FEBS Letters.\ This thoroughly revised and updated edition of Protein...
Search in google:
Praise for the First Edition of Protein Purification:"Contains much useful information for both the novice in protein purification and the experienced practitioner . . . [an] excellent practical manual." —Trends in Analytical Chemistry."[This book is] easy to read and gives a comprehensive description of all major methods of protein purification . . . a much welcomed source of information for protein biochemists." —FEBS Letters.This thoroughly revised and updated edition of Protein Purification provides state-of-the-art coverage of chromatographic and electrophoretic protein separation and characterization methods. Balancing theory, procedures, and applications, it offers professionals and students in biochemistry, organic chemistry, and analytical chemistry quick access to a wide range of important techniques, including:* Gel filtration and ion exchange chromatography* Affinity and covalent chromatography* Reversed phase and hydrophobic interaction chromatography* Electrophoresis in gels* Immunoelectrophoresis and capillary electrophoresis* Protein mapping by two-dimensional polyacrylamide gel electrophoresis* Protein elution and blotting methods* Large-scale electrophoresis processes. Booknews This second edition text offers a range of techniques of protein purification to professionals and students of biochemistry, analytic chemistry, and organic chemistry. After the introduction the book is divided into two main sections. The first covers chromatography and includes topics such as gel filtration, ion exchange and covalent chromatography, and affinity partitioning of proteins. The final section deals with electrophoresis, and features a new chapter on large-scale electrophoretic processes as well as completely rewritten chapters on protein mapping by two dimensional polyacrylamide electrophoresis, protein elution and blotting techniques, and capillary electrophoretic separations. Annotation c. by Book News, Inc., Portland, Or.
PrefaceContributors1Introduction to Protein Purification32Introduction to Chromatography433Gel Filtration794Ion Exchange Chromatography1455Chromatofocusing2076High-Resolution Reversed-Phase Chromatography2397Hydrophobic Interaction Chromatography2838Immobilized Metal Ion Affinity Chromatography3119Covalent Chromatography34310Affinity Chromatography37511Affinity Partitioning of Proteins Using Aqueous Two-Phase Systems44312Electrophoresis in Gels46313Isoelectric Focusing49514Immunoelectrophoresis52915Protein Mapping by Two-Dimensional Polyacrylamide Electrophoresis55716Protein Elution and Blotting Techniques58717Capillary Electrophoretic Separations60318Large-Scale Electrophoretic Processes651Index679
\ BooknewsThis second edition text offers a range of techniques of protein purification to professionals and students of biochemistry, analytic chemistry, and organic chemistry. After the introduction the book is divided into two main sections. The first covers chromatography and includes topics such as gel filtration, ion exchange and covalent chromatography, and affinity partitioning of proteins. The final section deals with electrophoresis, and features a new chapter on large-scale electrophoretic processes as well as completely rewritten chapters on protein mapping by two dimensional polyacrylamide electrophoresis, protein elution and blotting techniques, and capillary electrophoretic separations. Annotation c. by Book News, Inc., Portland, Or.\ \
