Technologies for Detection of DNA Damage and Mutations
''Useful and timely.'' —-Mutagenesis ''Of considerable value.'' —-Journal of Medical Genetics ''Quite readable....a comprehensive overview....perfectly covers the needs of those researchers who have to decide on the best strategy to identify damage or mutations at the molecular level.'' —-Trends in Cell Biology ''The formats of the presentations are uniform and ample and up-to-date references are provided at the end of each chapter...will be welcomed by postgraduate researchers of all ages...
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''Useful and timely.'' —-Mutagenesis ''Of considerable value.'' —-Journal of Medical Genetics ''Quite readable....a comprehensive overview....perfectly covers the needs of those researchers who have to decide on the best strategy to identify damage or mutations at the molecular level.'' —-Trends in Cell Biology ''The formats of the presentations are uniform and ample and up-to-date references are provided at the end of each chapter...will be welcomed by postgraduate researchers of all ages and should retain its usefulness for a long time.'' —-Endeavour, 21(4), 1997 This important resource thoroughly reviews a wide range of techniques used in mutagenesis research-ranging from established techniques to recently developed methodologies-based on the polymerase chain reaction. DNA damage analysis, DNA repair assays, and mutation detection are a few of the techniques featured. Chapters present detailed experimental protocols benefiting researchers and students in the fields of toxicology, biotechniques, molecular biology, photobiology, medical genetics, and oncology. Booknews A collection of 31 discussions developing techniques useful in mutagenesis research, highlighting new technologies advanced through the advent of the polymerase chain reaction. The majority of the contributors supply experimental protocols in addition to explanations of rationale in the areas of methods for analyzing DNA damage and repair, damage frequencies in single cells or cell populations, damage distribution along gene sequences, methods of screening and sequence analysis, and complete mammalian mutagenesis systems using selectable genes in cell culture and living animals. Annotation c. Book News, Inc., Portland, OR (booknews.com)
Ch. 1Microgel electrophoresis of DNA from Individual Cells: Principles and MethodologyCh. 2The Cytokinesis-Block Micronucleus TechniqueCh. 3Agarose Gel Electrophoresis for DNA Damage AnalysisCh. 4[superscript 32]P-Postlabeling for Detection of DNA AdductsCh. 5A Postlabeling Assay for Oxidative DamageCh. 6Radioimmunoassay of DNA Damaged by Ultraviolet LightCh. 7Monoclonal Antibody-Based Quantification and Repair Analysis of Specific Alkylation Products in DNACh. 8Detection of Oxidative DNA Base Damages: Immunochemical and Electrochemical ApproachesCh. 9Strategies for Measuring Damage and Repair in Gene-Sized Specific DNA SequencesCh. 10Methods to Measure the Repair of GenesCh. 11Mapping and Quantification of Bulky Chemical-Induced DNA Damage Using UvrABC NucleasesCh. 12The Use of DNA Glycosylases to Detect DNA DamageCh. 13PCR-Based Assays for the Detection and Quantitation of DNA Damage and RepairCh. 14DNA Damage Analysis Using an Automated DNA SequencerCh. 15Ligation-Mediated PCR for Analysis of UV DamageCh. 16Ligation-Mediated PCR for Analysis of Oxidative DNA DamageCh. 17Single-Strand Ligation PCR for Detection of DNA AdductsCh. 18Heteroduplex AnalysisCh. 19Mutation Analysis by Denaturing Gradient Gel Electrophoresis (DGGE)Ch. 20Constant Denaturant Gel Electrophoresis (CDGE) in Mutation ScreeningCh. 21Single-Strand Conformation Polymorphism AnalysisCh. 22Two-Dimensional Gene ScanningCh. 23Ligase Chain Reaction for the Detection of Specific DNA Sequences and Point MutationsCh. 24The Protein Truncation Test (PTT) for Rapid Detection of Translation-Terminating MutationsCh. 25Single Nucleotide Primer Extension for Analysis of Sequence VariantsCh. 26Sequencing of PCR ProductsCh. 27Detection and Characterization of Mutations in Mammalian Cells with the pSP189 Shuttle Vector SystemCh. 28The HPRT Gene as a Model Study for Mutation AnalysisCh. 29Bacteriophage Lambda and Plasmid lacZ Transgenic Mice for Studying Mutations in VivoCh. 30The Use of lacI Transgenic Mice in Genetic ToxicologyCh. 31Genotypic Mutation Assay (RFLP/PCR)Index
\ BooknewsA collection of 31 discussions developing techniques useful in mutagenesis research, highlighting new technologies advanced through the advent of the polymerase chain reaction. The majority of the contributors supply experimental protocols in addition to explanations of rationale in the areas of methods for analyzing DNA damage and repair, damage frequencies in single cells or cell populations, damage distribution along gene sequences, methods of screening and sequence analysis, and complete mammalian mutagenesis systems using selectable genes in cell culture and living animals. Annotation c. Book News, Inc., Portland, OR (booknews.com)\ \
