HPLC of Peptides and Proteins
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Hands-on experts from academia and industry comprehensively describe how to successfully perform all the critical HPLC techniques needed for the analysis of peptides and proteins. The methods range from commonly used techniques to those for capillary to large-scale preparative isolation. The authors have also presented a number of specific applications as case studies to illustrate the analytical approaches to a particular separation or assay challenge, with examples drawn from contemporary fields in biochemistry and biotechnology.
PrefaceContributors1HPLC of Peptides and Proteins: Basic Theory and Methodology32Reversed-Phase High-Performance Liquid Chromatography93Ion-Exchange Chromatography234High-Performance Hydrophobic Interaction Chromatography455Gel Filtration Chromatography556Hydrophilic Interaction Chromatography757Immobilized Metal Ion Affinity Chromatography of Proteins898Immunoaffinity Chromatography of Proteins1039Liquid Chromatography - Mass Spectrometry and Tandem Mass Spectrometry of Peptides and Proteins11110Capillary Separations14311Micropreparative HPLC of Peptides and Proteins16512Multidimensional HPLC Purification of Proteins17713Analytical High-Performance Liquid Chromatography18314Prep/Semiprep Separations of Peptides19115Large-Scale Protein Chromatography21116Proteolytic Peptide Mapping22717Mass Spectrometric Characterization of Posttranslationally Modified Proteins - Phosphorylation24518Analyses of Glycopeptides and Glycoproteins by Liquid Chromatography - Mass Spectrometry and Liquid Chromatography - Tandem Mass Spectrometry26319HPLC in the Analysis of Peptide Metabolism27520Isolation and Characterization of Naturally Processed MHC-Bound Peptides From the Surface of Antigen-Presenting Cells29121Australian Funnel-Web Spider Venom Analyzed With On-Line RP-HPLC Techniques30722HPLC and Mass Spectrometry of Intrinsic Membrane Proteins32323IgG Purification34124DNA-Binding Proteins: LC-MS to Identify Key Domains in RNA Polymerase - Promoter Interactions35125Sensitive Enzymatic Analysis of Histidine Decarboxylase Using HPLC36526Automated vs Manual Profiling of Peptide Libraries by Mass Spectrometry37727Proteome Analysis387Index401
