Introduction to Modern Liquid Chromatography
Search in google:
The latest edition of the authoritative reference to HPLC High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples. This carefully considered Third Edition maintains the strengths of the previous edition while significantly modifying its organization in light of recent research and experience. The text begins by introducing the reader to HPLC, its use in relation to other modern separation techniques, and its history, then leads into such specific topics as: The basis of HPLC separation and the general effects of different experimental conditions Equipment and detection The column—the "heart" of the HPLC system Reversed-phase separation, normal-phase chromatography, gradient elution, two-dimensional separation, and other techniques Computer simulation, qualitative and quantitative analysis, and method validation and quality control The separation of large molecules, including both biological and synthetic polymers Chiral separations, preparative separations, and sample preparation Systematic development of HPLC separations—new to this edition Troubleshooting tricks, techniques, and case studies for both equipment and chromatograms Designed to fulfill the needs of the full range of HPLC users, from novices to experts, Introduction to Modern Liquid Chromatography, Third Edition offers the most up-to-date, comprehensive, and accessible survey of HPLC methods and applications available.
Glossary of symbols and abbreviations1 Introduction 12 Basic Concepts and the Control of Separation 193 Equipment 874 Detection 1475 The Column 1996 Reversed-Phase Chromatography for Neutral Samples 2537 Ionic Samples: Reversed-Phase, Ion-Pair, and Ion-Exchange Chromatography 3038 Normal-Phase Chromatography 3619 Gradient Elution 40310 Computer-Assisted Method Development 47511 Qualitative and Quantitative Analysis 49912 Method Validation Michael Swartz Swartz, Michael Lloyd R. Synder Synder, Lloyd R. 53113 Biochemical and Synthetic Polymer Separations Timothy Wehr Wehr, Timothy Carl Scandella Scandella, Carl Peter Schoenmakers Schoenmakers, Peter Lloyd R. Synder Synder, Lloyd R. 56914 Enantiomer Separations Michael Lammerhofer Lammerhofer, Michael Nobert M. Maier Maier, Nobert M. Wolfgang Linder Linder, Wolfgang Lloyd R. Synder Synder, Lloyd R. 66515 Preparative Separations Geoff Cox Cox, Geoff Lloyd R. Synder Synder, Lloyd R. 72516 Sample Preparation Ronald Majors Majors, Ronald Lloyd R. Synder Synder, Lloyd R. 75717 Troubleshooting 809Appendix I Properties of HPLC Solvents 879Appendix II Preparing Buffered Mobile Phases 887Index 891
