Practical Methods for Biocatalysis and Biotransformations
Biocatalysts are increasingly used by chemists engaged in fine chemical synthesis within both industry and academia. Today, there exists a huge choice of high-tech enzymes and whole cell biocatalysts, which add enormously to the repertoire of synthetic possibilities.\ Practical Methods for Biocatalysis and Biotransformations is a “how-to” guide focussing on commercially available enzymes and strains of microorganisms that are readily obtained from culture collections. The source of starting...
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Biocatalysts are increasingly used by chemists engaged in fine chemical synthesis within both industry and academia. Today, there exists a huge choice of high-tech enzymes and whole cell biocatalysts, which add enormously to the repertoire of synthetic possibilities. Practical Methods for Biocatalysis and Biotransformations is a “how-to” guide focussing on commercially available enzymes and strains of microorganisms that are readily obtained from culture collections. The source of starting materials and reagents, hints, tips and safety advice (where appropriate) are given to ensure, as far as possible, that the procedures are reproducible. Comparisons to alternative methodology are given and relevant references to the primary literature are cited. Contents include:Introduction to the application of biocatalysts in the pharma industryintroduction to molecular biological techniques for biocatalyst developmentLipase and protease methods including enzyme supports and dynamic kinetic resolutionsNitrile hydrolases and hydratasesAlcohol dehydrogenases and co-factor recyclingEpoxide hydrolasesAmino acid synthesisWhole cell processesOther biocatalysts, including monoamineoxidases, aminotransterases, and lyases Practical Methods for Biocatalysis and Biotransformations is an essential text offering validated biocatalyst methods for researchers and postgraduate students in industry and academia.
1 Biotransformations in small-molecule pharmaceutical development 12 Biocatalyst identification and scale-up : molecular biology for chemists 833 Kinetic resolutions using biotransformations 1174 Dynamic kinetic resolution for the synthesis of esters, amides and acids using lipases 1335 Enzymatic selectivity in synthetic methods 1656 Aldolase enzymes for complex synthesis 2037 Enzymatic synthesis of glycosides and glucuronides 2278 Synthesis of cyanohydrins using hydroxynitrile lyases 2559 Synthesis of chiral sec-alcohols by ketone reduction 27310 Reduction of functional groups 29511 Enzymatic oxidation chemistry 31912 Whole-cell oxidations and dehalogenations 351Index 397
\ From the Publisher"Chapters cover key biotransformations from the literature, detailing the procedures used, the sources of the starting materials and reagents, references, and tips and advice, where appropriate." (JACS, 2010)\ "It starts with a review of the current use of biocatalysis in the pharmaceutical industry. Although this is focused on a large pharmaceutical company (GlaxoSmithKline), it covers a wide variety of aspects of the industry and offers insightful comparisons of biocatalysis with ‘traditional' organic synthesis." (Chemistry World, July 2010)\ "I can recommend to all those who are interesting in carrying out biotransformations in the laboratory, and the book should help persuade those who have never used a biotransformation in an organic synthesis, to try one for the first time." (Organic Process Research & Development Journal, 2010)\ \ \
