Cell Culture Technology for Pharmaceutical and Cell-Based Therapies
Edited by two of the most distinguished pioneers in genetic manipulation and bioprocess technology, this bestselling reference presents a comprehensive overview of current cell culture technology used in the pharmaceutical industry. Contributions from several leading researchers showcase the importance of gene discovery and genomic technology development in the production of biotechnology products, tissue engineering, and cell-based therapies. Offering detailed guidance, they cover the steps...
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Edited by two of the most distinguished pioneers in genetic manipulation and bioprocess technology, this bestselling reference presents a comprehensive overview of current cell culture technology used in the pharmaceutical industry. Contributions from several leading researchers showcase the importance of gene discovery and genomic technology development in the production of biotechnology products, tissue engineering, and cell-based therapies. Offering detailed guidance, they cover the steps leading up to the creation of viable therapies including host cell selection, cloning and gene amplification, bioreactor design and operation, protein purification, optimization, scale-up, and facility design. Doody Review Services Reviewer:Bruce A. Fenderson, PhD(Thomas Jefferson University)Description:Cell culture and protein purification are cornerstones of the pharmaceutical industry. They provide a means for drug discovery and the large-scale production of medicines. This comprehensive book includes 20 chapters on a wide range of tropics, including cell culture bioreactors, stem cells, protein glycosylation, product capture, and facility design. Purpose:According to the editors, the purpose of this book is to "cover cell culture bioprocessing from A to Z, starting from cell line development to the finished product. It encompasses both fundamental principles and practical aspects of the technology." The editors believe that the book will be a major resource on these topics for years to come.Audience:The book is written primarily for biochemists, cell biologists, materials scientists, and engineers. Research scientists and specialists in the pharmaceutical industries will be particularly interested in this book, owing to its depth of coverage of new methods and practical applications for drug development. Features:The book provides an encyclopedia of practical information on cell culture technology. The authors develop their topics in great detail and assume that the readers have familiarity with basic concepts in this field. For example, we learn that "spacing between impellers should be lower than 2/3T to retain strong interstage mass transfer." Each chapter includes an extensive bibliography.Assessment:The editors have brought together an outstanding group of experts from both private industry and academia. The topics include all major aspects of pharmaceutical product development based on modern principles of biochemistry, molecular biology and cell biology. The chapters are heavy reading. I recommend this book for basic scientists with a desire to learn more about methods of cell culture technology and product purification used in the pharmaceutical industry.
1Cell culture technology - an overview12Recombinant DNA technology and cell line development153Medium development414Cell metabolism815Protein glycosylation1136Cell culture bioreactors1557Aeration, mixing and hydrodynamics in bioreactors2258Instrumentation and process control2499Cell culture kinetics and modeling29910Fed-batch cultivation of mammalian cells for the production of recombinant proteins34911Optimization of high cell density perfusion bioreactors38712Cell separation and product capture41713Downstream processing43914Formulation, filling and packaging48315Validation of cell culture-based processes and qualification of associated equipment and facility52316Facility design55317Production of proteins by transient expression60518Principles and applications of the insect cell-baculovirus expression vector system62719Advances in adult stem cell culture69320Ex vivo culture of hematopoietic and mesenchymal stem cells for tissue engineering and cell-based therapies723
\ From The CriticsReviewer: Bruce A. Fenderson, PhD(Thomas Jefferson University)\ Description: Cell culture and protein purification are cornerstones of the pharmaceutical industry. They provide a means for drug discovery and the large-scale production of medicines. This comprehensive book includes 20 chapters on a wide range of tropics, including cell culture bioreactors, stem cells, protein glycosylation, product capture, and facility design. \ Purpose: According to the editors, the purpose of this book is to "cover cell culture bioprocessing from A to Z, starting from cell line development to the finished product. It encompasses both fundamental principles and practical aspects of the technology." The editors believe that the book will be a major resource on these topics for years to come.\ Audience: The book is written primarily for biochemists, cell biologists, materials scientists, and engineers. Research scientists and specialists in the pharmaceutical industries will be particularly interested in this book, owing to its depth of coverage of new methods and practical applications for drug development. \ Features: The book provides an encyclopedia of practical information on cell culture technology. The authors develop their topics in great detail and assume that the readers have familiarity with basic concepts in this field. For example, we learn that "spacing between impellers should be lower than 2/3T to retain strong interstage mass transfer." Each chapter includes an extensive bibliography.\ Assessment: The editors have brought together an outstanding group of experts from both private industry and academia. The topics include all major aspects of pharmaceutical product development based on modern principles of biochemistry, molecular biology and cell biology. The chapters are heavy reading. I recommend this book for basic scientists with a desire to learn more about methods of cell culture technology and product purification used in the pharmaceutical industry.\ \ \ 3 Stars from Doody\ \
