Protein Engineering and Design
Experimental protein engineering and computational protein design are broad but complementary strategies for developing proteins with altered or novel structural properties and biological functions. By describing cutting-edge advances in both of these fields, Protein Engineering and Design aims to cultivate a synergistic approach to protein science.\ Experimental Protein Engineering\ The first half of the book discusses experimental approaches to protein engineering and starts by describing...
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Experimental protein engineering and computational protein design are broad but complementary strategies for developing proteins with altered or novel structural properties and biological functions. By describing cutting-edge advances in both of these fields, Protein Engineering and Design aims to cultivate a synergistic approach to protein science.Experimental Protein EngineeringThe first half of the book discusses experimental approaches to protein engineering and starts by describing several high-throughput screening platforms for protein engineering. Key techniques used for diversity generation are also discussed. The next few chapters present examples of therapeutics, enzymes, biomaterials, and other proteins that have been engineered by rational or combinatorial approaches. The section finishes with a chapter on the use of non-natural amino acids in protein engineering.Computational Protein DesignThe second half of the book introduces computational protein design, beginning with a chapter on computational and informatics algorithms used in protein engineering. Core components of computational protein design are then discussed in detail, and examples of heuristic protein design are provided. Subsequent chapters present examples of how computational design has played a critical role in advancing the field of protein engineering. Concluding with a chapter outlining current challenges in the field, this book makes computational protein design and diversity-oriented protein engineering widely accessible to a broad audience in academia and industry alike.
Preface viiEditors ixContributor List xiChapter 1 Phage Display Systems for Protein Engineering Andreas Ernst Sachdev S. Sidhu 1Chapter 2 Cell Surface Display Systems for Protein Engineering Sarah J. Moore Mark J. Olsen Jennifer R. Cochran Frank V. Cochran 23Chapter 3 Cell-Free Display Systems for Protein Engineering Pamela A. Barendt Casim A. Sarkar 51Chapter 4 Library Construction for Protein Engineering Daa Lipovek Marco Mena Shaun M. Lippow Subhayu Basu Brian M. Baynes 83Chapter 5 Design and Engineering of Synthetic Binding Proteins Using Nonantibody Scaffolds Shohei Koide 109Chapter 6 Combinatorial Enzyme Engineering Patrick C. Cirino Christopher S. Frei 131Chapter 7 Engineering of Therapeutic Proteins Fei Wen Sheryl B. Rubin-Pitel Huimin Zhao 153Chapter 8 Protein Engineered Biomaterials Cheryl Wong Po Foo Sarah C. Heilshorn 179Chapter 9 Protein Engineering Using Noncanonical Amino Acids Deniz Yüksel Diren Pamuk Yulia Ivanova Krishna Kumar 205Chapter 10 Computer Graphics, Homology Modeling, and Bioinformatics David F. Green 223Chapter 11 Knowledge-Based Protein Design Michael A. Fisher Shona C. Patel Izhack Cherny Michael H. Hecht 239Chapter 12 Molecular Force Fields Patrice Koehl 257Chapter 13 Rotamer Libraries for Molecular Modeling and Design of Proteins Hidetoshi Kono 281Chapter 14 Search Algorithms Julia M. Shifman Menachem Fromer 293Chapter 15 Modulating Protein Structure M.S. Hanes T.M. Handel A.B. Chowdry 313Chapter 16 Modulation of Intrinsic Properties by Computational Design Vikas Nanda Fei Xu Daniel Hsieh 327Chapter 17 Modulating Protein Interactions by Rational and Computational Design Jonathan S. Marvin LorenL. Looger 343Chapter 18 Future Challenges of Computational Protein Design Eun Jung Choi Gurkan Guntas Brian Kuhlman 367Index 389
