Protein Purification: Design and Scale up of Downstream Processing
A comprehensive introduction to the art and science used by the biochemist and process engineer in the design and optimization of large-scale protein-purification processes. Covers the basics of proteins' properties, the unit operations involved in protein purification as well as important related issues of process design, integration with upstream operations, cost and timing to market. Includes an abundance of figures and tables.
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Protein purification describes general techniques useful for translating industrial bioprocesses from laboratory to any larger scale. This book is directed towards readers with both biochemistry and engineering backgrounds. One of the challenges in this field is the integration of engineering and biology in the design of process systems. The work is designed to facilitate the interaction between these two groups. Backed by practical examples, case studies and extensive literature references, the book offers primary value for technologists, whose specialty is the design and optimization of fermentation and cell culture processes for downstream recovery of protein and peptide products. Of use to researchers, production operators and students, Dr. Wheelwright's strategy sets forth practical guidelines and shows how to define product specifications and establish design strategies, specify unit operations, scale up unit operation recovery techniques, and optimize purification processes.
Process Design and Competitive Advantage.Strategies for Process Design.Protein Characteristics.Analytical Methods.Impact of Upstream Operations.Cell Disruption.Centrifugation.Precipitation and Extraction.Filtration.General Chromatography.Ion Exchange Chromatography.Hydrophobic Interaction Chromatography and Reversed Phase Chromatography.Affinity Chromatography.Gel Filtration Chromatography.Large-Scale Examples.Index.
